ISSN: 0973-7510

E-ISSN: 2581-690X

Yan Liu, Wei-Nan Yuan and Xuan-Wei Zhou

Plant Biotechnology Research Center, Key Laboratory of Urban Agriculture (South) Ministry of Agriculture, School of Agriculture and Biology, Shanghai Jiaotong University, Shanghai – 200240, China.
J. Pure Appl. Microbiol. 2014, 8(6):4245-4254
© The Author(s). 2014
Received: 20/07/2014 | Accepted: 06/09/2014 | Published: 31/12/2014
Abstract

This paper is concerned with optimization of submerged culture conditions for mycelial growth and bioactivity components production by Cordyceps militaris. Based on investigation of the most suitable carbon source and nitrogen source by one-factor-at-a-time method, the combination of carbon and nitrogen sources were optimized to investigate the effects on mycelial growth and bioactive components production using L9(34) orthogonal array. The effects of medium components on mycelial growth of C. militaris and adenosine production were in the order of soluble starch> dried silkworm chrysalis meal> fructose> soybean meal, and those on cordycepin production were in the order of fructose> soybean meal> dried silkworm chrysalis meal> soluble starch. The optimal medium combination, soluble starch 22.50 g/L, fructose 17.50 g/L, soybean meal 5.00 g/L and silkworm chrysalis meal 3.75 g/L, was beneficial for the accumulation of mycelial biomass (³2.36 g/L), while the optimal medium combination included soluble starch 12.50 g/L, fructose 22.50 g/L, soybean meal 3.75 g/L and silkworm chrysalis meal 3.75 g/L for the accumulation of adenosine (³193.67 µg/g), and soluble starch 12.50 g/L, fructose 17.50 g/L, soybean meal 3.75 g/L and silkworm chrysalis meal 5.00 g/L for the accumulation of cordycepin (³243.33 µg/g).

Keywords

Cordyceps militaris, Cordycepin, Adenosine, Mycelial biomass, L9(34)orthogonal array

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© The Author(s) 2014. Open Access. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License which permits unrestricted use, sharing, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.