LDM process is used for preparing three-dimensional scaffolds for tissue engineering rapid prototyping technologies. Because of its forming process is complex, influenced by a variety of factors caused by processing environment is not stable, the forming of scaffold pore size can not be guaranteed, forming precision is poor. So there is a need for forming parameters to optimize. Aim at LDM process characteristics, the paper determine the main process parameters that affect the bone scaffold forming quality. First, the influence degree of process parameters on one of indexes such as wire width precision and wire height precision is determined by orthogonal experiment. Then, in the problem of a number of process parameters optimization, using the degree of grey incidence method, the number of process indicators of the optimization problem is transformed into the single degree of gray correlation maximization problem, realizing multi-objective parameter optimization. The experiment results show that, using the combined optimization of technological parameters of preparation of bone scaffold, it’s forming quality is good, and it can well meet the stem cell growth conditions.