ISSN: 0973-7510
E-ISSN: 2581-690X
A cellulolytic fungal strain was successfully isolated from local region and further subjected to solid state fermentation (SSF). Optimization of production parameters were done at shake flask level to apply at production scale. Low cost approach was determined for commercial viability of such industrial enzyme. Basic as well as applied parameters were optimized for better production. The maximum activity were recorded as 2.9 U/mL, 8.3 U/mL and 110 U/mL for FPase, CMCase and b-glucosidase respectively at 30 C and pH 6 with wheat bran as substrate with 20% (w/w) inoculums concentration on 5th day of incubation. These parameters can further be used as benchmark for upscaling of the process for commercial production under SSF conditions.
Cellulases, Solid State Fermentation, Opimization, FPase, CMCase, β-glucosidase
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