ISSN: 0973-7510

E-ISSN: 2581-690X

Khosro Issazadeh , Nasrin Porghanbar Moghadam, Fatemeh Darabipor, Mojgan Hosseini and Narjes Mohammady Bandary
Department of Microbiology, Faculty of Basic Science, Lahijan Branch, Islamic Azad University, Lahijan, Iran.
J Pure Appl Microbiol. 2015;9(1):281-284
© The Author(s). 2015
Received: 25/05/2014| Accepted: 14/07/2014| Published: 31/03/2015
Abstract

Poly –hydroxyl butyrate( PHB) is a natural,biodegradable polymer accumulated in the form of intracellular granules by a large variety of bacteria. It is among the most investigated biodegradable polymer in recent years. The PHBs are non-toxic, biocompatible and biodegradable thermoplastics  that can be produced from renewable resources. The study aimed at screening and identifying a potential PHB accumulating B.megaterium PTCC 1656 and optimization of media parameters for increased PHB production by this strain bioplastics. For this study B.megaterium PTCC 1656 was purchased  from the fungi and bacteria collection center of the Iranian science and technology research organization. An their PHB production was determined under different conditions such as incubation time, Carbon and Nitrogen source. The result showed that , glucose was the best carbon source and in this conditions the maximum production of PHB was 0.42 g/l at 45h. PHB production starts in response to stress imposed on cell usually by nitrogen limitation,although in the presence of abundant carbon source. Actually PHB production was dependent on nutrient limitation.

Keywords

Bacillus megaterium, Poly-hydroxyl butyrate, Optimization

Article Metrics

Article View: 736

Share This Article

© The Author(s) 2015. Open Access. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License which permits unrestricted use, sharing, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.