ISSN: 0973-7510

E-ISSN: 2581-690X

R. Thyagarajan , S. Karthick Raja Namasivayam and G. Narendra Kumar
1Department of Biotechnology, Sathyabama University, Chennai,Tamilnadu, India.
J Pure Appl Microbiol. 2014;8(3):2485-2490
© The Author(s). 2014
Received: 24/07/2013 | Accepted: 09/10/2013 | Published: 31/06/2014

In monogastric animals the ability of digesting phytase is lacking which hydrolysis phytate to release phosphate. This paper concentrates on the commercial production of phytase which replenish the need of phosphate in livestock feed production. Response surface methodology was applied for the optimization of phytase production by Hypocrea lixii SURT01. Four factors such as carbon source (sucrose), nitrogen source (peptone), substrate (phytate) in different concentration and pH were optimized using central composite design (CCD). The optimum levels of variables that supported maximum enzyme activity were 1.5% sucrose, 0.2% phytate, 3% peptone and pH 7.5. The validity of the model in optimized conditions was verified. With this composition, the phytase production was 74.56IU (average of three repeats) after 96 h of cultivation, while the predicted maximum production was 73.42 IU. The optimized medium resulted in significant increase of the phytase yield by Hypocrea lixii in shake-flask cultivation.


Phytase, Hypocrea lixii SURT01, Central composite design, Response surface methodology

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© The Author(s) 2014. Open Access. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License which permits unrestricted use, sharing, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.