ISSN: 0973-7510

E-ISSN: 2581-690X

S. Karthick Raja Namasivayam and R.S. Arvind Bharani
1Department of Biotechnology, Sathyabama University, Chennai, Tamil Nadu, India.
J. Pure Appl. Microbiol. 2014, 8(6):4867-4875
© The Author(s). 2014
Received: 06/04/2014 | Accepted: 19/08/2014 | Published: 31/12/2014
Abstract

Nomuraea rileyi (Farlow) Samson is an important fungal biocontrol agent and extensively used  as mycoinsecticide against economic important insect pests in various parts of the world. Success of biocontrol agent is primarily based on the production of adequate quantity of inoculam using suitable media.Supply of nutrients is an important factor which influences the biomass production.Statistical optimization strategy as response surface methodology (RSM) is adopted to maximize the biomass production through submerged and solid state fermentation. RSM can identify the various interactions among different parameters and it has been extensively applied for optimization of cultural medium conditions and other process parameters in bio processes In the  present study, Central composition design (CCD) was used for optimizing the parameters. Temperature (X­1­), pH (X2), Maltose (X3), Peptone (X4) was chosen as independent variables. The maximum percentage of biomass yield is obtained at 3.87g/mL of maltose and peptone at 0.55g/mL,temperature 28.1ºC and pH 7.09.Metabolites were extracted from the culture filtrate of fungi grown in media and showed distinct effect on the life stages of Spodoptera litura. Lethal time 50(LT 50) and lethal concentration 50 (LC 50) was highly influenced by larval stages and concentration.

Keywords

Nomuraea rileyi, Spodoptera litura, Response surface methodology, Metabolites

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