ISSN: 0973-7510

E-ISSN: 2581-690X

Azratul Ashimah Nur Mohd Dom and Faridah Yusof

1Department of Biotechnology Engineering, Faculty of Engineering, International Islamic University Malaysia, P.O. Box 10, 50728 Kuala Lumpur, Malaysia.
J. Pure Appl. Microbiol. 2014, 8(6):4465-4469
© The Author(s). 2014
Received: 20/08/2014 | Accepted: 27/10/2014 | Published: 31/12/2014

Tetrathionate hydrolase produced by Thiobacillus ferrooxidans has been known to be the enzyme responsible in the devulcanization of used rubber prior to recycling.  The aim of this study was to statistically obtain a model that would yield an optimized media concentrations for the best production of this bacterial enzyme.  One-factor-at-a-time (OFAT) experimental design was applied to find the possible optimum range of five media components. Based on the OFAT results, a face centered central composite design (FCCCD) by Design Expert® 6.0.8 was applied using a 4-factors, 3-center points to find the optimum media concentrations to yield the highest tetrathionate hydrolase. Response Surface Methodology (RSM) predicted the level of factors for maximized response. In this study, it is concluded that the medium containing 0.4g/L MgSO4.7H2O, 4.0g/L (NH4)2SO4, 2.0g/L Na2S2O3.5H2O and 3.0 g/L KH2PO4 results in the production of the highest specific activity of tetrathionate hydrolase (295.7 U/mg Protein).  Other parametric conditions used were 0.25g/L for CaCl2.2H2O with an initial pH buffer of 4, incubated at 25oC while agitating at a speed of 125 rpm.


Thiobacillusferrooxidans, tetrathionate hydrolase, OFAT, FCCCD, devulcanization of rubber

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© The Author(s) 2014. Open Access. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License which permits unrestricted use, sharing, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.