ISSN: 0973-7510

E-ISSN: 2581-690X

K.B. Uppuluri1-2 and D.S. Rami Reddy2
1Department for Biotechnology, Bapatla Engineering College, Bapatla – 522 101, India.
2Center for Biotechnology, Department of Chemical Engineering, Andhra University, Visakhapatnam – 530 017, India.
J Pure Appl Microbiol. 2009;3(1):83-90
© The Author(s). 2009
Received: 05/12/2008 | Accepted: 01/01/2009| Published: 30/04/2009

Acute lymphocytic leukemia is a common leukemia characterized by frequent infections and anemia. Thousands of new cases are diagnosed each year worldwide. L-Asparaginase (E.C., also known as L-asparagine amino hydrolase, is a potential anti-tumor enzyme that catalyses the hydrolysis of L-asparagine into L-aspartic acid and ammonia. L-Asparaginase production was investigated in the isolated filamentous fungi on sesame cake using solid state fermentation (SSF). L-Asparaginase production using isolated Aspergillus niger grown on sesame cake has been optimized in a column bioreactor using a statistically-based method. The 23  BHH   design having   eight   different   fermentation   conditions   was   applied to evaluate their significance on L-Asparaginase activity, where the three independent variables evaluated were  Aeration,  Thickness  of  Solid  Substrate  Bed  and  Fermentation  Temperature. Aeration and Temperature were identified to be important variables and had a positive effect on  responses,  however,  the  thickness  of  solid  substrate  had  an  insignificant  effect  on  L-Asparaginase activity  within the tested range. The optimum fermentation condition for L-Asparaginase activity consisted of high aeration (0.4 vvm), deeper thickness of bed (22 cm) and high fermentation temperature (32°C), respectively. Under optimum levels, a maximum L-Asparaginase activity of 344.2132 IU was predicted which is slightly less than the activity at batch level.


L-Asparaginase, Aspergillus niger, Sesame cake, Solid-state fermentation, column bioreactor, Box Hunter & Hunter design

Article Metrics

Article View: 0

Share This Article

© The Author(s) 2009. Open Access. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License which permits unrestricted use, sharing, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.