ISSN: 0973-7510

E-ISSN: 2581-690X

Zhang De-Yong1 , Xu Xiao-Lu1, Shen Xiu-Ying2, Lu Yin1, Xu Hui-Ying1 and Hao Fei-Lin1
1College of Biology and Environmental Engineering, Zhejiang Shuren University, Hangzhou, China.
2College of Biology and Chemical Engineering, Zhejiang University of Science and Technology, Hangzhou, China.
J Pure Appl Microbiol. 2013;7(Spl. Edn.: November):727-733
© The Author(s). 2013
Received: 27/09/2013 | Accepted: 04/11/2013 | Published: 30/11/2013

Nucleocapsid protein(N) of avian coronavirus infectious bronchitis virus(IBV) plays an important role in maintaining viral structure and replication. The N protein produced in vitro can be applied in the fields of diagnosis, vaccination and scientific research. Expression of IBV N protein in a prokaryotic expression system has been established before, but the medium used is complex and expensive. To optimize the fermentation conditions with simpler medium, the Plackett-Burman experiment was designed for factor screening, and then response surface methodology was applied to optimize the fermentation conditions. The medium was optimized as 0.86 % polypeptone, 1.14 % yeast extract, 1.63 % NaCl, 0.02 % L-arabinose and pH 7.0. Five batches of fermentation according to the above conditions resulted in a mean value of 44.25% for the relative N protein quantity, with a 0.14 % deviation from theoretically deduced value. The fermentation conditions optimized in this study is appropriate for N protein production, which stablely expresses IBV N protein and gives a high yield.


IBV, N gene, Expression in vitro, Plackett-Burman experiment, Response surface methodology

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© The Author(s) 2013. Open Access. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License which permits unrestricted use, sharing, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.