The present study is to optimize and produce gallic acid of plant tannin under solid state fermentation of different process parameters like temperature, pH of the medium, agitation speed (RPM), volume of Innoculum (ml), moisture, fermentation time, tannic acid, ammonium nitrate, sucrose, substrate influencing gallic acid production from plant tannin were carried out by Minitab TM 15 software. A systematic study with RSM based on FFD and CCD at 95% confidence level was used to study the interactions among the different variables of fermentation process to maximize the gallic acid production. Under the optimized cultivation conditions A. tubingenesis synthesized 94.34% was produced at 35°C temperature, pH 5.5, 96hrs fermentation period, 300mM ammonium nitrate, 600mg sucrose, 916.291mM tannic acid from plant tannin under solid state fermentation. Model validations at optimum operating conditions showed excellent agreement between the experimental results and the predicted responses. Thus the present fungal species to be efficient for gallic acid production. The present study demonstrates the pomegranate peels as a significant substrate statistical optimization with major parameters for gallic acid production can be highly applicable for industrial scale further.
Full factorial design, Central composite design, Aspergillus tubingenesis, Pomegranate
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