Studies were under taken on Aspergillus oryzae SCBRD11 isolated from the soil sample using potato dextrose agar and starch used as a substrate to evaluate their ability to produce amylase. The amylase producers detected by the clear zone around the colony by simple plate assay method. Among the fifteen isolates Aspergillus oryzae SCBRD11 is the potential strain. The amylase synthesis were increased their yield after the optimization of fermentation parameters. The optimum pH 4.5, temperature 31⁰C and inoculum size 1.0 ml. This enzyme was growth associated.