ISSN: 0973-7510

E-ISSN: 2581-690X

Noura El-Ahmady El-Naggar1 , Attiya Hamed Mohamedin2, A.A. Sherief2 and Suzan Mohamed Hussien2
1Department of Bioprocess Development, Genetic Engineering and Biotechnology Research Institute, City of Scientific Research and Technological Applications, Alexandria, Egypt.
2Department of Botany, Faculty of Science, Mansoura University, Egypt.
J. Pure Appl. Microbiol., 2016, 10 (1): 823-839
© The Author(s). 2016
Received: 30/11/2015 | Accepted: 15/01/2016 | Published: 31/03/2016
Abstract

The genus Nocardiopsis, a widespread group in phylum Actinobacteria, has received much attention owing to its ecological diversity and ability to produce a rich array of bioactive metabolites. Nocardiopsis sp. strain SH89 was isolated from soil sample collected from Borg El Arab city, Alexandria, Egypt  and tested for its ability to produce bioactive compounds. According to the cultural, morphological, biochemical characteristics and 16S rRNA sequencing, the isolated strain is identified as Nocardiopsis chromatogenes strain SH89 and its sequencing product was deposited in the GenBank database under accession number KT236083. The Plackett–Burman statistical design was used for initial screening of fifteen different factors for their significance on bioactive metabolites production by Nocardiopsis chromatogenes strain SH89. Among the variables screened, KNO3, medium volume and agitation speed had significant effects on bioactive metabolites production. The levels of these significant variables and their interaction effects were optimized by Box–Behnken statistical design. An overall one-fold increase in the production of antimicrobial metabolites was achieved after optimization by using response surface methodology compared with that obtained in the un-optimized medium. This study points the success of statistical model in developing an optimized production medium for enhanced antibacterial compound production by Nocardiopsis chromatogenes strain SH89.

Keywords

Nocardiopsis sp., Bioactive metabolites, Fermentation, Plackett-Burman design, Box-Behnken design, 16S rRNA, identification.

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© The Author(s) 2016. Open Access. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License which permits unrestricted use, sharing, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.