ISSN: 0973-7510

E-ISSN: 2581-690X

D. Das, S.B. Mohapatra and P. Ray
1Department of Microbiology, College of Basic Science and Humanities, Orissa University of Agriculture and Technology, Bhubaneswar-751003, Odisha, India.
J Pure Appl Microbiol. 2015;9(Spl. Edn. 2):475-485
© The Author(s). 2015
Received: 09/03/2015 | Accepted: 15/04/2015 | Published: 30/11/2015
Abstract

Biosurfactants are amphiphilic compounds produced on living surfaces, mainly on surfaces of microorganisms or secreted extracellularly. Out of twenty isolates obtained from Bhitarkanika mangrove forest, five were positive for biosurfactant production. Biosurfactant production (mg/ml) and surface tension reduction (mN/m) on different days of incubation of all the five isolates when considered, only one isolate (BMSed II) was found to produce the maximum biosurfactant & highest surface tension reduction on 7 days of incubation in comparison to others. On optimization of different parameters (2% inoculum, 2% glucose, 37°C temperature and pH – 8) on potent isolate BMSed II was found to be suitable for maximum biosurfactant production of 2.14g/l. The 16S rDNA gene sequence of (BMSed II) was submitted to NCBI gene bank and found similar to Bacillus altitudinis (Accession number KT005170) by phylogenetic analysis. The biosurfactant produced by Bacillus altitudinis was characterized as a lipopeptide using Thin-layer chromatography (TLC), Fourier transform infrared spectroscopy (FTIR) and H1 Nuclear magnetic resonance (NMR) analysis.

Keywords

Biosurfactant, Bacillus altitudinis, lipopeptide, TLC, FTIR, NMR

Article Metrics

Article View: 1045

Share This Article

© The Author(s) 2015. Open Access. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License which permits unrestricted use, sharing, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.