ISSN: 0973-7510

E-ISSN: 2581-690X

N. Utharalakshmi1 , A. Ganesh Kumar2 and G. Narendrakumar1
1Department of Biotechnology, Faculty of Bio and Chemical Engineering, Sathyabama University, Chennai – 600119, India.
2National Institute of Ocean Technology, NIOT Campus, Chennai – 600 100, India.
J Pure Appl Microbiol. 2014;8(Spl. Edn. 2):713-719
© The Author(s). 2014
Received: 06/09/2014 | Accepted: 13/10/2014 | Published: 30/11/2014
Abstract

This study was aimed to isolate and screen the cellulolytic ability of fungi from litter soil samples collected in and around Chennai, India. The culture conditions were optimized for the growth of fungi by SSF using Rice Bran. Fungi are the main cellulase producing microorganisms when compared with other microorganisms. Among eleven fungal isolates, a fungal isolates were identified as maximum cellulase producer and labelled as SB4. The cultures were maintained on PDA slants at 4°C. The morphology was studied by LPCB method and fungal identification was done based on 18s rRNA ITS sequencing. The zone of clearance was observed on CMC agar plates after 72 hours of incubation by staining with 1% Congo red. The zone of hydrolysis  was found to be high for SB4 having the highest diameter of 17 mm. Maximum cellulase production for rice bran was observed to be high at initial moisture 85%, pH-6, temperature-31°C and fermentation period was 14th day. The enzyme production was analyzed individually by Dinitrosalicylic acid (DNS) method. The cellulosic degradation was confirmed by FTIR and SEM on 0th day and 28th day.

Keywords

Cellulase, Aspergillus flavus, Solid state Fermentation, FTIR, SEM

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© The Author(s) 2014. Open Access. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License which permits unrestricted use, sharing, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.