ISSN: 0973-7510

E-ISSN: 2581-690X

J. Meenupriya1 and Thangaraj2
1Department of Biotechnology, Sathyabama University, Chennai – 600 119, India.
2C.A.S. in Marine Biology, Annamalai university, Parangipettai – 608 502, India.
J Pure Appl Microbiol. 2014;8(1):179-183
© The Author(s). 2014
Received: 09/01/2013 | Accepted: 27/02/2013 | Published: 28/02/2014
Abstract

This study aims to characterize and optimize the xylanases production using marine sponge – derived Aspergillus terreus MP1. A.terreus showed optimal enzyme activity on day 6 while sucrose and urea were used as  carbon source and nitrogen source respectively. The enzyme was purified by  dialysis and the purified enzyme exhibited optimal activity at pH 5.6 in ambient temperature of 55ºC . Zymogram analysis displayed four activity bands corresponding to xynalolytic activity and molecular weight was determined to be 66, 45, 35 and 19 kDa. The optimized Xylanase was also utilized effectively as an environmental friendly technique of dye degradation process.

Keywords

Microbial Xylanase, Aspergillus terreus, LSF, SSF, Deinking, Dye degradation

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