This study aims to characterize and optimize the xylanases production using marine sponge – derived Aspergillus terreus MP1. A.terreus showed optimal enzyme activity on day 6 while sucrose and urea were used as carbon source and nitrogen source respectively. The enzyme was purified by dialysis and the purified enzyme exhibited optimal activity at pH 5.6 in ambient temperature of 55ºC . Zymogram analysis displayed four activity bands corresponding to xynalolytic activity and molecular weight was determined to be 66, 45, 35 and 19 kDa. The optimized Xylanase was also utilized effectively as an environmental friendly technique of dye degradation process.
Microbial Xylanase, Aspergillus terreus, LSF, SSF, Deinking, Dye degradation
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