Two emu chicks in the age of 4-5 weeks died after showing shivering and dullness. Post Mortem examination revealed hepatomegaly, granulomatous lesions in lungs and congestion of the intestine. Clinical materials such as trachea, heart, lung, liver, kidney, intestine and gizzard were collected and subjected to bacterial isolation. Eschericia coli could be isolated from all the specimens collected from both dead emu chicks and the cases were confirmed as colibacillosis. All the E. coli isolates were subjected to antibiotic sensitivity test and were found to be sensitive to Enrofloxacin, Ciprofloxacin and Ofloxacin and resistant to Penicillin, Ampicillin, Amoxycillin, Amoxyclav, Erythromycin, Streptomycin, Gentamicin,  Doxycycline, Oxytetracycline  and  Tetracycline.