A novel actinomycete, strain NEAE 85, was isolated from a soil sample collected from Alwaraq, Egypt. This strain shows high L-asparaginase activity and subjected to taxonomic analyses. It showed a range of chemical and morphological properties consistent with its classification in the genus Nocardiopsis. The strain formed well developed substrate mycelium, aerial hyphae are long, moderately branched, straight to flexuous, or irregularly zigzagged or spiral forms, different spirals are wrapped together to form synnemata. The mycelia of a synnema fragment in later stages to form bacillary spores of various lengths. Phylogenetic analysis using 16S rRNA gene sequences showed that the isolate was closely related to Nocardiopsis baichengensis strain YIM 90130 (87% 16S rRNA gene sequence similarity) and Nocardiopsis trehalosi strain VKM Ac-942 (88%).  However, a comparative study between strain NEAE-85 and its closest phylogenetic neighbours of the genus Nocardiopsis revealed significant differences between them in morphological, cultural, and physiological characteristics. It is evident that strain NEAE-85 clearly represents a novel species of the genus Nocardiopsis, for which the name Nocardiopsis synnemasporogenes NEAE-85 sp. nov. is proposed and sequencing product was deposited in the GenBank database under accession number KJ200340.