Molecular biology studies based on PCR amplification require the extraction of good quality genomic DNA. In this study, freeze- sudden thawing as a safety, new, quick and low-cost genomic DNA extraction technique from gram-negative and gram-positive bacteria, and Saccharomyces and non- Saccharomyces yeasts for PCR applications has been developed. This technique does not require any risky chemicals, enzymes and/or extra purification steps. DNA extracted by this method is suitable for PCR applications using random and specific primers such as RAPD-PCR for fingerprinting and DNA sequencing of the 16S rDNA or the D1/D2 domain of the 26S rDNA for molecular identification of bacterial and yeast isolates respectively.
Freeze-sudden thawing, Safety and rapid DNA extraction
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