ISSN: 0973-7510

E-ISSN: 2581-690X

Abd El-Latif Hesham1,2
1Microbial Genetics and Environmental Meta-Genome Biotechnology Genetics Department, Faculty of Agriculture, Assiut University, Assiut 71526, Egypt.
2Biology Department, Faculty of Science, King Khalid University, Abha, Saudi Arabia.
J Pure Appl Microbiol. 2014;8(1):383-388
© The Author(s). 2014
Received: 30/11/2013 | Accepted: 01/01/2014 | Published: 28/02/2014
Abstract

Molecular biology studies based on PCR amplification  require the extraction  of good quality genomic DNA. In this study, freeze- sudden thawing as a safety,  new, quick and low-cost genomic DNA extraction technique from gram-negative and gram-positive bacteria, and Saccharomyces and non- Saccharomyces yeasts for PCR applications has been developed. This technique does not require any risky chemicals, enzymes and/or extra purification steps. DNA extracted by this method is suitable for PCR applications using random and specific primers such as RAPD-PCR for fingerprinting and  DNA sequencing of  the 16S rDNA or the D1/D2 domain of the 26S rDNA for molecular identification of bacterial and yeast  isolates respectively.

Keywords

Freeze-sudden thawing, Safety and rapid DNA extraction

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© The Author(s) 2014. Open Access. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License which permits unrestricted use, sharing, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.