ISSN: 0973-7510

E-ISSN: 2581-690X

Hai Yan Li1,2
1Shandong Provincial Key Laboratory of Functional Macromolecular Biophysics, Dezhou University, 566 University Road West, Dezhou – 253 023, China.
2Department of Physics, Dezhou University, 566 University Road West, Dezhou – 253 023, China.
J Pure Appl Microbiol. 2013;7(Spl. Edn.: November):647-654
© The Author(s). 2013
Received: 27/09/2013 | Accepted: 04/11/2013 | Published: 30/11/2013
Abstract

p53 DNA-binding domain (p53DBD) plays crucial roles in domain stability, DNA binding and dimerization. In this article, we analyze the motion mode of p53DBD using the Gaussian network model and anisotropy elastic network model. For each structure, the calculated B-factors by two models obtain good agree with the experimentally determined B-factors in x-ray crystal structures. The results show that the four CRs (conserved regions) in the p53DBD have small fluctuation in the slowest mode of GNM, but the same regions lay the peaks of the fast modes. With the aid of the anisotropy elastic network mode, we analyze the motion directions of this domain. The first slowest mode of ANM for both structures mainly corresponds to the rotational motion. However, the CR V (N-terminal) shows an opposite direction with the other parts of structure. Through this motion, p53DBD will bind to its consensus sequence DNA in a highly cooperative manner. By analyzing cross-correlations between residue fluctuations of p53DBD, the results indicate that residues involved in similar functions are fluctuating in a cooperative manner.

Keywords

p53 DNA-binding domain (p53DBD), Motion Mode, Elastic Network Model

Article Metrics

Article View: 0

Share This Article

© The Author(s) 2013. Open Access. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License which permits unrestricted use, sharing, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.