ISSN: 0973-7510

E-ISSN: 2581-690X

Anuradha Singh , Mukesh Srivastava, Mohammad Shahid, Sonika Pandey, Shubha Trivedi and Yatindra Kumar
Biocontrol Laboratory, Department of Plant Pathology, Chandra Shekhar Azad University of Agriculture & Technology, Kanpur – 208 002, India.
J. Pure Appl. Microbiol., 2016, 10 (1): 431-436
© The Author(s). 2016
Received: 21/11/2015 | Accepted: 13/01/2016 | Published: 31/03/2016
Abstract

Most isolates of the genus Trichoderma were found to act as mycoparasites of the many economically important aerial and soil-borne plant pathogens. Trichoderma has gained importance as an alternate for chemical pesticides and hence an attempt was intended to corroborate the positive connexion of molecular and morphological characters. A fungal strain of Tr(CSAU)/7284  (Trichoderma reesei) was isolated from a soil sample collected from CSA Farm, Kanpur, state, India. The universal primers were used for amplification of 25SrRNA sequence fragment and therefore the strain was characterized by using 25SrRNA gene sequence with the association of ITS marker. It’s planned that the identified strain Trichoderma reesei be assigned because the kind strain of a species of the genus Trichoderma supported by phylogenetic tree analysis along with the 25SrRNA gene sequence search in Ribosomal Database Project, small subunit 232rRNA and large subunit rRNA databases. The sequence was deposited in GenBank with the accession number KM999966. Thus an integrated approach of morphological and molecular markers will be used to spot a superior strain of Trichoderma for its industrial exploitation.

Keywords

Biocontrol Agent, Internal Transcribed Spacer, Trichoderma, 25S ribosomal RNA gene.

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© The Author(s) 2016. Open Access. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License which permits unrestricted use, sharing, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.