Mosquito-borne diseases are still a major human and animal health problem in the world.  Identification of mosquito vectors is important in many respects including development of vector control strategies. Adults and immature stages of Ae.aegypti were identified based on the morphological characters using light and electron microscope and appropriate illustration (pictorial) keys. It is common knowledge that morphological ID is not as accurate as molecular ID. Recently DNA-based identification methods using molecular markers such as nuclear ribosomal internal transcribed spacer (ITS), cytochrome b (Cyt-b) and cytochrome c oxidase subunit 1 and 2 COI, COII  which become an important method to differentiate between siblings and closely related species of mosquitoes. Adults of  Ae. aegypti were collected from five areas in Jeddah city. Molecular identification by isolating both of DNA and total protein of  Ae. aegypti   were conducted. The results showed  that DNA was isolated from mature and immature stages of Ae.aegypti  using PCR  technique  from  either the lab strain or from older and/or field specimens. This assay consisted of different primers reaction, which could amplify the DNA of both mature and immature stages  producing fragment of three distinct sizes,  ~1250 bp  , 500 bp , ~ 400-600 bp and 300bp  for  CO1B , Cryb,  ITS2  and  ITS2-2  respectively  Some of these loci were sequenced  and submitted to the GenBank database NCBI  Protein samples of (larva, pupa and adult ) of  the dengue fever vector,  Ae. aegypti   were isolated. The relative molecular weight of the detected bands was approximately in the range of 16.6 – 75.6 kDa.