ISSN: 0973-7510

E-ISSN: 2581-690X

S. Chithra and Oommen P. Saj
Department of Botany and Research Centre, University College, Thiruvanthapuram – 34, India.
J Pure Appl Microbiol. 2010;4(1):397-400
© The Author(s). 2010
Received: 11/09/2009 | Accepted: 25/10/2009 | Published: 30/04/2010
Abstract

Andrographis echioides (L.) Nees  is an erect annual herb under the family Acanthaceae. The plant is seen as an invasive weed in tropical areas.  The whole plant has medicinal properties hence widely exploited that creates  threat to its existence. Only a few  studies were done on this plant. This work is an attempt  to reveal  the  advantages of callus cultures in the propogation and to evaluate   the antibacterial activity  of both the  leaf  and  the callus extract. For tissue culture studies  different  hormone combinations were used  to observe  the profuse callus growth. NAA/ BA  combinations showed  profuse callus growth. For antibacterial studies three bacterial strains were used. Only the leaf extracts  showed the inhibition where as no response was noticed  in the case of the callus extracts. Based on the present studies, it is concluded  that  tissue culture is an easy method to protect and conserve the plant  from  extinction  and the callus culture extracts  are less efficient as  an  antibacterial  agent.

Keywords

Andrographis echioides, tissue culture, callus, leaf extract, antibacterial  activities

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