ISSN: 0973-7510

E-ISSN: 2581-690X

Abhishek Mishra , Mohammad Shahid, Supriya Dixit, Sonika Pandey, Mukesh Srivastava and Ved Ratan
Biocontrol Laboratory, Department of Plant Pathology, Chandra Shekhar Azad University of Agriculture & Technology, Kanpur – 208 002, Uttar Pradesh, India.
J. Pure Appl. Microbiol., 2016, 10 (2): 1243-1250
© The Author(s). 2016
Received: 13/01/2016 | Accepted: 19/04/2016 | Published: 30/06/2016
Abstract

Most isolates of the genus Trichoderma were found to act as mycoparasites of many economically important aerial and soil-borne plant pathogens.Trichoderma has gained importance as a substitute for chemical pesticides and hence an attempt was intended to corroborate the positive relatedness of molecular and morphological characters. A fungal strain of Trichoderma asperellum CA-03/9840 was isolated from a soil sample collected from Farmer Field, Sultanpur ,UttarPradesh, India. The universal primers were used for amplification of 5.8SrRNA gene fragment and the strain was characterized by using 5.8SrRNA gene sequence with the help of ITS marker. It is proposed that the identified strain Trichoderma asperellum CA-03/9840 be assigned as the type strain of a species of the genus Trichodermabased on     TrichoKey analysis together with the 5.8SrRNA gene sequence search in Ribosomal Database Project, small subunit rRNA and large subunit rRNA databases. The sequence was deposited in GenBank with the accession number KU821782. Thus an integrated approach of morphological and molecular markers can be employed to identify a superior strain of Trichoderma for its commercial exploitation.

Keywords

5.8S ribosomal RNA gene, Trichoderma, ITS, Biocontrol, Antagonist.

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© The Author(s) 2016. Open Access. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License which permits unrestricted use, sharing, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.