The present study reports the biodegradation studies of Monocrotophos in agricultural soils of Visakhapatnam district. Bacterial strains were isolated from agricultural soils of Visakhapatnam dist. The isolates based on their morphological, biochemical and molecular characteristics were identified as Bacillus aryabhatta Strain ENP 3, Bacillus drentensis strain ENA1, Bacillus firmus Strain EN5C, and Staphylococcus vitulinus Strain EN5F. These four strains were isolated for the first time in this region. The degradation potential of the organisms for Monocrotophos as the only carbon source were studied and also the influence of Cow dung manure and NPK fertilizer on the growth and degradation capacity of organisms were studied. Degradation potential in media for individual organism as well as consortia was studied using spectrophotometric analysis. LC-MS was used to detect the degradation potential of microbial consortia in soil and also know the intermediate metabolites formed during degradation. It was found that the bacterial consortium has the highest degradation potential compared to individual organisms and Bacillus aryabhattai strain ENP3 has higher is degradation potential for Monocrotophos compared to other organisms.