ISSN: 0973-7510

E-ISSN: 2581-690X

Research Article | Open Access

Husham Hachim Mohammed1 , Mohammed Flayyih Tareef2 and Ali Kamal Mohammed3

1,2Department of medical laboratory science, College of Health and Medical Technology, University of Middle Technical, Baghdad-Iraq.
3Bacteriology Section, Central Teaching Hospital of Pediatric, Ministry of Health, Baghdad-Iraq.
J Pure Appl Microbiol. 2018;12(4):2087-2094 | Article Number: 5322
Received: 17/10/2018 | Accepted: 30/11/2018 | Published: 30/12/2018
Abstract

The aim of this present study to was to detect the 16S rRNA gene sequences of Escherichia coli local isolate from dialysis patients and to compare their genetic relatedness utilizing phylogenetic analysis. A total of (75) clinical samples (Urine) were collected from in dialysis patients but found thirty specimens of E. coli admitted at Baghdad Medical City, Baghdad Teaching Hospital during the period from October, 2017 to January, 2018 platinum calibrated sterile wire loop to transfer 1ml of the uncentrifuged urine specimen and streaked on MacConkey agar incubated at 37°C for 18-24 hours. The results of phylogenetic analysis showed that Proximity and the genetic dimension among themselves and the world with more than 98% to 99%compatibility values.

Keywords

E. coli, UTI, 16S rRNA gene

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