ISSN: 0973-7510

E-ISSN: 2581-690X

Yashpal S. Malik1 , Vinita Rawat2, Kuldeep Sharma1, Naveen Kumar1, Nirupama Vaid1, Ritu Rakholia3 and Balasubramanian Ganesh4
1Division of Biological Standardization, Indian Veterinary Research Institute (IVRI),
Izatnagar – 243 122, India.
2Department of Microbiology, Government Medical College, Haldwani, India.
3Department of Pediatrics, Government Medical College, Haldwani, India.
4Division of Virology, National Institute of Cholera and Enteric Diseases (NICED) (ICMR), Kolkata, India.
J Pure Appl Microbiol. 2014;8(1):733-737
© The Author(s). 2014
Received: 15/10/2013 | Accepted: 12/12/2013 | Published: 28/02/2014

Acute diarrhea caused by an array of infectious agents is the most important disease of childhood with high mortality worldwide. Multiple enteric infections are serious threat with fatal outcome compared to infection by one causative agent. Initial identification of those multiple infections is essential for formulating the better treatment recipes and preventive measures in long term. Therefore we describe here such infection in an infant with acute gastroenteritis from Haldwani city, Uttarakhand, India. The molecular investigations were carried out for the common enteric infection viz. rotavirus, astrovirus, calicivirus and picobirnavirus by RT-PCR, cloning and sequencing followed by phylogenetic analysis. The investigation detected the presence of group A (RVA) and B (RVB) rotaviruses with picobirnavirus (PBV) simultaneously. Further, RVA was genotyped as P[6] and PBV as of genogroup I. Phylogenetic analysis clustered RVA, RVB and PBV with American bovine group A isolate, human Myanmar isolate and Chinese porcine isolate, respectively suggestive of respective species origin. This report is the first to describe a concomitant infection of PBV [genogroup I] with RVA and RVB. The investigation emphasizes that the presence of mixed infections should always be kept in mind in enteric infections with great demand of a reliable test that detects the presence of RVA, RVB and PBV simultaneously.


Viral Gastroenteritis, Concurrent Infection, RVA, RVB, PBV, Molecular Characterization

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