ISSN: 0973-7510

E-ISSN: 2581-690X

Research Article | Open Access
Selvaraj Stephen1   , Jothimani Pradeep1 , Chinnadurai Rajkumar2 , Rupal Samal3 , Ashwini Vishalakshi3 , Seetesh Ghose3 , Sendhil Coumary3 , Syed Habebullah3 and Balanehru Subramanian2
Department of Microbiology1, 3Obstetrics & Gynaecology, Mahatma Gandhi Medical College & Research Institute and 2Central Interdisciplinary Research Facility (CIDRF), Sri Balaji Vidyapeeth (SBV), Deemed-to-be-University, Puducherry – 607402, India.
J Pure Appl Microbiol, 2019, 13 (3): 1791-1796 | Article Number: 5801 | © The Author(s). 2019
Received: 01/08/2019 | Accepted: 08/09/2019 | Published: 23/09/2019

This study relates to the prevalence of common non-viral sexually transmitted infections in symptomatic women of Puducherry. Endocervical swabs were collected from 41 symptomatic women attending as outpatients in OB&G department of Mahatma Gandhi Medical College Hospital, Puducherry, using special swabs (eSwab) and transported to Microbiology department. DNA was extracted from these swabs and real time multiplex PCR was performed using FTD Urethritis plus in Rotor-Gene Q series 3000 (QIAGEN, Germany). The kit targets seven pathogens, viz., Chlamydia trachomatis, Neisseria gonorrhoea, Trichomonas vaginalis, Mycoplasma hominis, M. genitalium, Ureaplasma urealyticum and U. parvum. Among 41 symptomatic women, 17 were positive for single pathogen (U.parvum-11, U.urealyticum-4 and M.hominis-2) and six patients were positive for two or more pathogens. Interestingly, none of the patients were positive for genital chlamydiasis and gonorrhea. The significance of U.parvum as a potential pathogen needs to be confirmed. There was no statistical difference between the positive and negative patients among the different age groups.


Chlamydia trachomatis, Neisseria gonorrhoea, Trichomonas vaginalis, Mycoplasma hominis, M. genitalium, Ureaplasma.

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© The Author(s) 2019. Open Access. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License which permits unrestricted use, sharing, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.