ISSN: 0973-7510
E-ISSN: 2581-690X
J. Pure Appl. Microbiol. 2014, 8(6):4971-4973
© The Author(s). 2014
Received: 06/08/2014 | Accepted: 10/09/2014 | Published: 31/12/2014
Abstract
The DNA of whitefly transmitted (WFT) geminivirus was amplified from a viral template present in infected leaves by Polymerase Chain Reaction (PCR) by using degernative primers (PBL1v2040 and PCRc154)that amplify viral DNA-B fragment of approximately500-650bpfor detection of mungbean yellow mosaic virus.
Keywords
Legumes, Polymerase Chain Reaction, degenerate primers
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