ISSN: 0973-7510

E-ISSN: 2581-690X

Hossein Goudarzi1, Elnaz Sadat Mirsamadi1 , Zohreh Ghalavand1, Mojdeh Hakemi Vala1, Hamed Mirjalali2,3, Ali Hashemi1 and Ehsan Ghasemi1
1Department of Microbiology, Faculty of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
2Foodborne and Waterborne Diseases Research Center, Research institute for Gastroentrology and Liver Diseases, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
3Gastroenterology and Liver Diseases Research Center, Research institute for Gastroentrolog and Liver Diseases, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
J Pure Appl Microbiol. 2015;9(Spl. Edn. 2):145-151
© The Author(s). 2015
Received: 10/07/2015 | Accepted: 09/09/2015 | Published: 30/11/2015

Acinetobacter baumannii is an opportunistic pathogen causing infections in hospitalized patients, particularly in intensive care units (ICUs). The treatment of these infections is one of the most important concerns of physicians due to multidrug-resistant. The b-lactam antimicrobial drugs are choice treatment of infections caused by A. baumannii. The specimens included CSF (cerebrospinal fluid), wound, sputum, urine, BAL (Broncho Alveolar Lavage), blood and so on were taken from 128 patients who were hospitalized in several wards. The antimicrobial susceptibility test was carried out using kirby-bauer disc diffusion method for Piperacillin-Tazobactam, Ceftriaxon, Ceftazidime, Cefepime, Imipenem, Doripenem, Ertapenem, Cefotaxime, Ampicillin-Sulbactam antibiotics for all isolates. DNA of isolates was extracted and primers were designed and as well as amplification was carried out for blaIMP, blaVIM, blaNDM1 genes. The resistance to all discs used in this study was seen in all isolates. The Highest and the lowest rate of resistance were seen in Piperacillin-tazobactam and Ampicillin-sulbactam, 100% and 21% isolates, respectively. The results of PCR of blaIMP, blaVIM and blaNDM genes were as follows: blaIMP 5/128 (3.9%), blaVIM 9/128(7.03%) and blaNDM 0/128(0%). The highest sensitivity to antibiotics group was seen in Ampicillin-Sulbactam groups and also blaVIM was the most prevalent gene of resistance among Metallo-Beta-Lactamase genes in Acinetobacter baumannii isolates.


Iran, Acinetobacter baumannii, Polymerase Chain Reaction (PCR), Metallo-Beta-Lactamase

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© The Author(s) 2015. Open Access. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License which permits unrestricted use, sharing, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.