ISSN: 0973-7510

E-ISSN: 2581-690X

Research Article | Open Access
Md. Atik Mas-ud1#, Md Roushan Ali1,2#, S M Zia Hasan1,3,
Md Ashikul Islam1, Md. Faruk Hasan1, Md. Asadul Islam1 and Biswanath Sikdar1
1Professor Joarder DNA and Chromosome Research Lab., Department of Genetic Engineering and Biotechnology, University of Rajshahi, Rajshahi-6205, Bangladesh.
2School of Life Sciences and Medical Center, University of Science and Technology of China (USTC), 230027 Hefei, Anhui, China.
3Department of Neuroscience, School of Medicine, Memorial University of Newfoundland, St. John’s, NL, A1C 5S7, Canada.
J. Pure Appl. Microbiol., 2020, 14 (1): 147-156 | Article Number: 5976
https://doi.org/10.22207/JPAM.14.1.16 | © The Author(s). 2020
Received: 03/12/2019 | Accepted: 06/02/2020 | Published: 31/03/2020
Abstract

Human hair dandruff (HHD) is a common unwanted scalp disorder that is prevalent to most human populations all over the world. This study was designed to isolate and characterize pathogens that are responsible for HHD as well as the evaluation of their biological control technique. Isolated bacteria were characterized by different biochemical tests and molecular identification methods. Here, disc diffusion methods were used to determine antibiotic and antibacterial activity against isolated bacteria. The isolated bacterial colonies were found to be Gram-positive, small, round-shaped, and purple. PCR amplification was done using 27F and 1492R primer pairs. A BlastN search of a sequenced 1465 bp region of 16S rRNA in NCBI GenBank revealed approximately 99% genome similarity with Staphylococcus aureus. The sequence was deposited in GenBank (Accession No. MH603394). In the antibiotic sensitivity test, Kanamycin showed the highest 31.0±0.5 mm diameter zone of inhibition (DZI) against the isolated bacteria. Moreover, as a plant-derived compound, the Methanol extract of Allium sativum revealed the highest, 15.0±0.5 mm DZI. The present study would give a promising direction of identification and control of this pathogen biologically.

Keywords

Human hair dandruff, Molecular detection, Biological control, Staphylococcus aureus.

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