ISSN: 0973-7510

E-ISSN: 2581-690X

Sangram Ramane1, Rishendra Verma2, Tista Mondal1 and Vikramaditya Upmanyu3
1Mycobacteria Laboratory, Division of Bacteriology and Mycology, Indian Veterinary Research Institute (IVRI), Izatnagar, Bareilly – 243 122, India.
2Center for Animal Disease Research And Diagnosis (CADRAD) Indian Veterinary Research
Institute (IVRI), Izatnagar, Bareilly – 243 122, India.
3Scientist, Division of Biological Standardization, Indian Veterinary Research
Institute (IVRI), Izatnagar, Bareilly – 243 122, India.
J Pure Appl Microbiol. 2014;8(4):3279-3283
© The Author(s). 2014
Received: 18/02/2014 | Accepted: 21/04/2014 | Published: 31/08/2014
Abstract

Mycobacterium bovis 3/86 strain isolated from cattle was characterized based on RD region encodedMb3904, Mb3905 and Mb2002c gene sequences. PCR was performed to amplify Mb3904, Mb3905 and Mb2002c genes. Restriction enzymes digested amplified geneswere cloned in compatible pET vector and sequenced with vector specific primers. The sequenced genes and its deduced amino acid sequences were compared with the published sequences of reference strains. The sequences of the Mb3904, Mb3905 and Mb2002c genes share 99.6 to 100% nucleotide homology and 99.5 to 100% deduced protein sequence homology for all studied genes with published reference mycobacterial strains indicating their conserved nature.

Keywords

Mycobacterium bovis, Mb3904 gene, Mb3905 gene, Mb2002c gene, sequencing

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