ISSN: 0973-7510

E-ISSN: 2581-690X

Research Article | Open Access

Mohammed S. Al-Mogbel1, Fauwaz Al-Rashid2,3, Mamdoh Meqdam1, Hisham Al-Ajlan4 and Mushtaq A. Khan1

1Molecular Diagnostic and Personalized Therapeutics Unit, College of Applied Medical Sciences, University of Ha’il, Kingdom of Saudi Arabia.
2King Khalid Hospital, Ha’il, Kingdom of Saudi Arabia.
3College of Medicine, University of Ha’il, Kingdom of Saudi Arabia.
4Prince Sultan Military Medical City, Riyadh, Kingdom of Saudi Arabia.
J Pure Appl Microbiol, 2019, 13 (1): 265-269 | Article Number: 5373 | © The Author(s). 2019 

Received: 27/12/2018| Accepted: 03/02/2019 | Published: 19/03/2019

Staphylococcus aureus, including methicillin resistant S. aureus (MRSA) is the most commonly isolated pathogen in hospitals worldwide. The aim of present study was molecular characterization of Staphylococcus aureus isolated from renal hemodialysis (HD) patients from Ha’il region of Saudi Arabia. A total of 392 samples were screened from 204 HD patients for colonization of S. aureus. The isolated bacteria were identified by MALDI-TOF-MS. Antibiotic susceptibility testing was performed using Microscan. Among these isolates, 72 S. aureus (43% MRSA and 57% MSSA) were identified.The isolates were considerably resistant with varied profile to the antibiotics tested except being 100% susceptible to vancomycin, linezolid and teicoplanin. Of the isolates, 22.2% were positive for biofilm assay. Four representative MRSA isolates were selected and whole genome sequence analysis was performed using MiSeq. Two out of the 4 MRSA were found to be ST-1 and 2 were found to be ST-32. Among MRSA isolates, 25.8% were negative for mecA and all of them were negative for mecC gene. A high prevalence of MRSA in HD patients as well as high percentage of biofilm production in MRSA isolates highlights the vital role for standardized surveillance along with validated molecular typing methods to evaluate the incidence of MRSA and accordingly to control its spread.


Hemodialysis, Staphylococcus aureus, Whole genome sequencing Pathogenic bacteria, MALDI-  TOF-MS.

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© The Author(s) 2019. Open Access. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License which permits unrestricted use, sharing, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.