A total of 135 samples from 6 different fermented food sources were used to evaluate the presence of bacteriocin producing lactic acid bacteria. 100 strains of lactic acid bacteria isolated from the samples were screened for antibacterial activity against Staphylococcus aureus NCIM2079, Bacillus subtilis NCIM2106, Listeria monocytogenes MTCC 657, Enterococcus faecalis MTCC 9845 and Salmonella typhi NHRC 001. The potential LAB strains showing activity against pathogenic organisms were identified as Leuconostoc mesenetroides VITMS1, Leuconostoc mesenetroides VITMS4, Pediococcus pentosaceus VITMS7, Enterococcus mundtii MVS1 and interestingly MVP5 strain was found to be Uncultured bacterium clone ncd36c11c1. Leuconostoc mesenetroides VITMS4, Pediococcus pentosaceus VITMS7 showed high antibacterial activity against Listeria monocytogenes with 1280 AU/mL and Staphylococcus aureus with 1280 and 640 AU/mL. Suggesting that these strains can be exploited for the production of potential bacteriocins to inhibit food borne pathogens.
Lactic acid bacteria, Screening, 16S rRNA, Antibacterial activity, Arbitrary units
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