In this study, the open reading frame of a Secretory Antigenic Target (Esat) gene from M. tuberculosis in Escherichia coli BL21 (DE3) was cloned. Esat gene that was isolated from pulmonary TB patient was cloned into a plasmid vector (pGEM-Teasy) to construct pMB38. The E.coli DE3 clone carrying pMb38 was selected on X-gal medium. The expression of ORF in Esat gene was mediated using pRoExHTc under the control of Tre promoter and E.coli DE3 as host. Characterization of clones with recombinant plasmid was cut with restriction enzymes and sequencing of DNA inserts. Our results by BLAST analysis of sequencing showed 100% homology. It is hoped these proteins can then be used as an antigen for serological detection of latent TB in people.
ESAT gene; Clone; M. tuberculosis; E.coli
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