ISSN: 0973-7510

E-ISSN: 2581-690X

Shiva Hosseini1, Abbas Maleki1, Sobhan Ghafourian1, Mohammad Reza Fadavi1, Hasan Valadbeigi1, Parasto Shahmir1, Hossein Kazemi1, Rashid Ramazanzadeh2, Sara Soheili3, Iraj Pakzad1 and Nourkhoda Sadeghifard1
1Clinical Microbiology Research Center, Ilam University of Medical Sciences, Ilam, Iran.
2Microbiology Department, Faculty of Medicine, Kurdistan University of Medical Sciences, Sanandaj, Iran.
3Department of Medical Microbiology and Parasitology, Faculty of Medicicne and Health Sciences, Universiti Putra Malaysia, Malaysia.
J Pure Appl Microbiol. 2014;8(3):2217-2220
© The Author(s). 2014
Received: 27/07/2013 | Accepted: 19/09/2013 | Published: 31/06/2014

The common use of beta lactam antibiotics for tratment of bacteria infections leads to increase the world wide microbial resistance by producing beta lactamase enzyme among clinical isolates . In recent years, the production of broad-spectrum beta-lactamase enzymes in clinical isolates , especially E.coli and Klebsiella bacteria are common.  Typical Ampc enzymes (class C- ESBLs) confirm resistance to most oxyimino cephalosporins. The aim of this study is to determine the prevalence of AmpC type extended spectrum beta lactamases genes in clinical isolates of Klebsiella pneumoniae. 108 clinical sample of K.pneumoniae, isolated from hospitalized patients procured from two hospitals in Ilam and Tehran. To  identify Ampc genes, PCR method was used.  95/3 percent of isolates were resistant to Cefoxitin , 49 isolates were positive for FOXM cluster genes , 35 were positive for DHAM cluster genes and 6 were positive for CITM cluster genes. Our results showed that among of  clinical isolates of Klebsiella pneumoniae, prevalence broad-spectrum beta-lactamase enzymes and Ampc genes are relatively high.


Klebsiella pneumoniae, Beta-lactamase broad-spectrum, Ampc genes

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© The Author(s) 2014. Open Access. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License which permits unrestricted use, sharing, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.