Molecular Characterization of AmpC β-Lactamases among Klebsiella pneumoniae Isolated from Ilam and Tehran Hospitals, from Iran

ISSN: 0973-7510

E-ISSN: 2581-690X

Molecular Characterization of AmpC β-Lactamases among Klebsiella pneumoniae Isolated from Ilam and Tehran Hospitals, from Iran

Shiva Hosseini¹, Abbas Maleki¹, Sobhan Ghafourian¹, Mohammad Reza Fadavi¹, Hasan Valadbeigi¹, Parasto Shahmir¹, Hossein Kazemi¹, Rashid Ramazanzadeh², Sara Soheili³, Iraj Pakzad¹ and Nourkhoda Sadeghifard¹

¹Clinical Microbiology Research Center, Ilam University of Medical Sciences, Ilam, Iran.

²Microbiology Department, Faculty of Medicine, Kurdistan University of Medical Sciences, Sanandaj, Iran.

³Department of Medical Microbiology and Parasitology, Faculty of Medicicne and Health Sciences, Universiti Putra Malaysia, Malaysia.

J Pure Appl Microbiol. 2014;8(3):2217-2220

Received: 27/07/2013 | Accepted: 19/09/2013 | Published: 31/06/2014

Abstract

The common use of beta lactam antibiotics for tratment of bacteria infections leads to increase the world wide microbial resistance by producing beta lactamase enzyme among clinical isolates . In recent years, the production of broad-spectrum beta-lactamase enzymes in clinical isolates , especially E.coli and Klebsiella bacteria are common. Typical Ampc enzymes (class C- ESBLs) confirm resistance to most oxyimino cephalosporins. The aim of this study is to determine the prevalence of AmpC type extended spectrum beta lactamases genes in clinical isolates of Klebsiella pneumoniae. 108 clinical sample of K.pneumoniae, isolated from hospitalized patients procured from two hospitals in Ilam and Tehran. To identify Ampc genes, PCR method was used. 95/3 percent of isolates were resistant to Cefoxitin , 49 isolates were positive for FOXM cluster genes , 35 were positive for DHAM cluster genes and 6 were positive for CITM cluster genes. Our results showed that among of clinical isolates of Klebsiella pneumoniae, prevalence broad-spectrum beta-lactamase enzymes and Ampc genes are relatively high.

Keywords

Klebsiella pneumoniae, Beta-lactamase broad-spectrum, Ampc genes

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