p. aeruginosa, an opportunistic pathogen produces antimicrobial substance in the form of a diffusible pigment, pyocyanin. The pyocyanin pigment was extracted using solvent extraction and further characterization studies were performed. The genotypic confirmation of biosynthetic pyocyanin phz A gene was amplified by PCR using suitable primers. The amplified gene corresponded to 217bp sequence. Further, the antifungal activity of the pigment pyocyanin was assessed using various fungal (yeasts) cultures. The antifungal activity of the pigment was found to contain an inhibitory substance, pyrrolnitrin that helps to arrest the electron transport chain of the tested cultures. This pigment can be tailored for an enhanced antimicrobial efficiency targeting only the pathogenic micro organisms.
Pseudomonas, Pyocyanin, phz A
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