ISSN: 0973-7510
E-ISSN: 2581-690X
Candida albicans is a widespread opportunistic pathogen in immuno-compromised patients. ATPase activity and protein concentration using spectrophotometric method and antifungal activity by Microtitre / ELISA technique in terms of Minimum Inhibitory Concentration (MIC) was studied to understand the pattern of Candida albicans. Minimum Inhibitory Concentration for Clotrimazole and Nystatin showed 6.25µg/ml and 8.0µg/ml ‘’respectively’’. At optimum pH (7.0) and temperature (280C), 100% activity of ATPase was recorded. pH showed inverse relationship while temperature showed no remarkable variation of activity. ATPase was found to be 5.0% reduced by Clotrimazole, whereas Nystatin caused 39% inhibition at the optimum pH and temperature.
Candida albicans, Candidiasis, ATPase, Nystatin and Clotrimazole
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