ISSN: 0973-7510

E-ISSN: 2581-690X

Research Article | Open Access
Luma FM. Al-Ethafa1 , Ahlam AS. Al-Galebi2 and Mithal KA. Al-Hassani2
1Environmental Health Department, College of Environmental Science, Al-Qasim Green University, Iraq.
 2Department of Biological Science, College of Education, Al-Qadisiyah University, Iraq.
J Pure Appl Microbiol, 2019, 13 (3): 1745-1751 | Article Number: 5625 | © The Author(s). 2019
Received: 17/05/2019 | Accepted: 22/07/2019 | Published: 15/09/2019

The aim of the present study was to detect the prevalence of Anaplasma marginale in buffaloes in two Iraqi governorates, Al-Qadisiyah and Babylon, by the microscopy as well as the competitive-ELISA that used firstly among the Iraqi buffaloes. A total of 184 buffaloes from both sexes of different age groups of animals were submitted for collection of blood samples to prepare the smears and sera. Overall results were revealed on 10.33% and 36.41% positive animals by microscopy and competitive-ELISA, respectively. In addition, positive rates by both tests were 7.61%; by microscopy only, 2.72%; and by competitive-ELISA only, 28.8%. In Al-Qadisiyah and Babylon governorates, respectively, 8.7% and 11.96% of microscopy samples, and 44.57% and 28.26% of competitive-ELISA were positives with significant differences (P>0.05). Regarding to age factor, the highest prevalence was detected by microscopy in young age group (1-3 years) was 14.15%, whereas by competitive-ELISA, it was 55% in adult buffaloes group (>3 years). Significant increases (P>0.05) in rates of infection were showed in females compared to males, respectively, by microscopy (11.18% and 4.35%) and competitive-ELISA (39.13% and 17.39%).


Anaplasma marginale, Buffaloes, Microscopy, Competitive-ELISA, Iraq.

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© The Author(s) 2019. Open Access. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License which permits unrestricted use, sharing, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.