ISSN: 0973-7510

E-ISSN: 2581-690X

Research Article | Open Access
I. Gubert Joseph1, Selvaraj Stephen2 , N. Shivananda1 and Thiruvenkada Krishnan1
1Aravind Eye Hospital and Post Graduate Institute, Pondicherry, India.
2Department of Microbiology, Mahatma Gandhi Medical College & Research Institute, Sri Balaji Vidyapeeth (Deemed – to – be – University), Pondicherry, India.
J. Pure Appl. Microbiol., 2020, 14 (1): 591-594 | Article Number: 6082 | © The Author(s). 2020
Received: 10/02/2020 | Accepted: 29/02/2020 | Published: 07/03/2020

Fungal keratitis is one of the causes of severe ocular morbidity and blindness. The prevalence of fungal keratitis has been increased from 7–63%. Culture is mandatory for an Ophthalmologist to guide treatment. The main objective of this study is to identify the etiological agents of Mycotic keratitis in corneal ulcer patients based on the microscopy. A prospective study was conducted with a total of 200 corneal scrapings were collected during the period of January to Setember 2015. Direct smear examination was followed by culture on blood agar and Sabraoud Dextrose agar and incubated at 37°C for 48 hours (BA) and 25°C for seven days (SDA) 48 hrs . The cultures are stored in sterile distilled water. Fungus was isolated from 79 (39.5%) corneal ulcer patients. Out of which 32 (40.5%) were positive with 10% KOH. In the present study, 85% fungal ulcers were diagnosed in female patients and 15% in male. Among 79 fungus, Aspergillus species, Fusarium species, Curvularia species and unidentified dematiaceous fungi were isolated from corneal ulcers. The bacterial agents were identified in 10 patients (5.0%) by direct gram stain as well as culture. Females are the most prevalent for corneal ulcers. Diagnosis of fungal keratitis patients occurs mostly with Fusarium. It suggests that the most of our corneal ulcer patients are infected with the Fusarium. Another risk factor is contact lens for microbial keratitis at the adult age group in Pondicherry. Majority of the fungal keratitis infections have responded to voriconazole for medical management.


Fungal keratitis, Aspergillus, Fusarium, Potassium hydroxide, Slide culture technique.

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© The Author(s) 2020. Open Access. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License which permits unrestricted use, sharing, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.