Resistant bacteria in various sections of hospital as a main issue of infection transmission is undeniable. Thereby, to achieve infection disease control on hospital, bacteria recognizing and antibiotic resistance determination is necessary. This study is aimed to determine the pattern of microbial resistant of isolates that isolated from surgery ward and ICU and compare it with osocomial infection isolates. In a descriptive and cross-sectional study, during 6 months sampling was carried out from devices and ersonnel of surgery and ICU ward. In same period Nosocomial infections from surgery and ICU wards were cultured.  Bacterial on Standard criteria according to CLSI were diagnosed. Antibiotic susceptibility were done according to Kirby-Bauer Disk Diffusion .After culturing 130 samples were positive, the most frequency of isolated bacteria in both wards were Staphylococcus saprophyticus. Most frequent bacteria in ICU and Surgery ward involved;  Entrobacter (35%) and E.coli (25%), in a row. In nosocomial infections, frequency of samples that cultured were positive included in; Lesion (45%), Sputum(42.5%) and Urine(12.5%).  E.coli, Klebsiella and Staphylococcus aureus isolated from ICU shown high resistance to Amplicillin, Ceftazidime/Cefotaxime , Ceftriaxone, respectively. Whereas bacteria that isolated from Surgery ward shown high resistance to Tetracycline, Amoxicillin- Tetracycline, Ceftriaxone. Regarding to resistance pattern , there is a correlation between resistance of species that isolated from personnel and devices with nasocomial isolates. Therefore, it could be concluded that devices and personnel have main role to disseminate infection. Thereby, the proper disease control policy could be so useful to combat with issue.