ISSN: 0973-7510

E-ISSN: 2581-690X

Mirta Azizi1, Iraj Pakzad1,4 , Zahra Karam Nejad2,3, Fariba Khodayari2,3, Reza Azizian1,4, Farid Azizi Jalilian4, Morevat Taherikalani4, Masoud Hamidi5 and Reza Pakzad5
1Clinical Microbiology Research Center, Ilam University of Medical Sciences, Iran.
2Student Research Committee, Ilam University of Medical Sciences, Iran.
3Department Nursing, Faculty of Nursing and Midwifery, Ilam University of Medical Sciences, Iran.
4Department Microbiology, Faculty of Medicine, Ilam University of Medical Sciences, Ilam, Iran.
5Department of Epidemiology, Medical School, Tehran University of Medical Sciences, Tehran, Iran.
J Pure Appl Microbiol. 2014;8(2):1059-1064
© The Author(s). 2014
Received: 11/06/2013 | Accepted: 20/08/2013 | Published: 31/04/2014
Abstract

Resistant bacteria in various sections of hospital as a main issue of infection transmission is undeniable. Thereby, to achieve infection disease control on hospital, bacteria recognizing and antibiotic resistance determination is necessary. This study is aimed to determine the pattern of microbial resistant of isolates that isolated from surgery ward and ICU and compare it with osocomial infection isolates. In a descriptive and cross-sectional study, during 6 months sampling was carried out from devices and ersonnel of surgery and ICU ward. In same period Nosocomial infections from surgery and ICU wards were cultured.  Bacterial on Standard criteria according to CLSI were diagnosed. Antibiotic susceptibility were done according to Kirby-Bauer Disk Diffusion .After culturing 130 samples were positive, the most frequency of isolated bacteria in both wards were Staphylococcus saprophyticus. Most frequent bacteria in ICU and Surgery ward involved;  Entrobacter (35%) and E.coli (25%), in a row. In nosocomial infections, frequency of samples that cultured were positive included in; Lesion (45%), Sputum(42.5%) and Urine(12.5%).  E.coli, Klebsiella and Staphylococcus aureus isolated from ICU shown high resistance to Amplicillin, Ceftazidime/Cefotaxime , Ceftriaxone, respectively. Whereas bacteria that isolated from Surgery ward shown high resistance to Tetracycline, Amoxicillin- Tetracycline, Ceftriaxone. Regarding to resistance pattern , there is a correlation between resistance of species that isolated from personnel and devices with nasocomial isolates. Therefore, it could be concluded that devices and personnel have main role to disseminate infection. Thereby, the proper disease control policy could be so useful to combat with issue.

Keywords

Antibiotic susceptibility, Resistant Bacteria, ICU, Surgery, Hospital acquired infection

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© The Author(s) 2014. Open Access. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License which permits unrestricted use, sharing, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.