Due to reports of food borne illness associated with the consumption of fruit juices at several places in India and elsewhere, a study was undertaken to assess the safety of street vended fruit juices particularly sugar cane juice as possible sources of bacterial pathogens. Bacteriological examination of sugar cane juice was done by multiple-tube fermentation test. In the present investigation freshly squeezed juices of sugarcane, showed occurrence of high microbial loads consisting of number of pathogens like Coliforms, Fecal coliforms, E.coli, S.aureus. The presence of E.coli in water is a strong indication of recent sewage or animal waste contamination. Sewage may contain many other types of disease-causing organisms. This can be prevented by checking the water source of the vendors.