ISSN: 0973-7510

E-ISSN: 2581-690X

Sulaiman Ali AL Yousef
1Department of Medical Laboratory Sciences, College of Applied Medical Science, University of Dammam, P.O. Box 1683 Hafr Al Batin – 31991, Saudi Arabia.
J Pure Appl Microbiol. 2013;7(2):939-945
© The Author(s). 2013
Received: 11/02/2013 | Accepted: 05/04/2013 | Published: 30/06/2013
Abstract

Among 27 Staphylococcus aureus isolates, only 3 were methicillin-resistant
S. aureus
(MRSA) and 24 were Methicillin sensitivity S. aureus (MSSA). Phages specific for  MRSA were isolated from collected soil samples. The spot test and single plaque technique were used for detection and biological purification of phages. Bacteriophages of  MRSA found to be limited occurrence in soils from where the samples had be taken. Two single plaques (A and B) having different morphology were picked up as a single phage isolates. The titers were 6.4 x 108 pfu ml-1 and 1.7 x 1010 pfh ml-1 for phage isolates A and B, respectively. The optimum pH and thermal inactivation point were found to be 9, 6 and 75, 65°C for A and B isolates, respectively. Biologically characterization and morphology of phage particles confirmed two phage types, that can designated as phage MRSA-A and phage MRSA-B.

Keywords

MRSA- phage, The spot test, Single plaque technique, Phage titer, Particle morphology

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