ISSN: 0973-7510
E-ISSN: 2581-690X
Among 27 Staphylococcus aureus isolates, only 3 were methicillin-resistant
S. aureus (MRSA) and 24 were Methicillin sensitivity S. aureus (MSSA). Phages specific for MRSA were isolated from collected soil samples. The spot test and single plaque technique were used for detection and biological purification of phages. Bacteriophages of MRSA found to be limited occurrence in soils from where the samples had be taken. Two single plaques (A and B) having different morphology were picked up as a single phage isolates. The titers were 6.4 x 108 pfu ml-1 and 1.7 x 1010 pfh ml-1 for phage isolates A and B, respectively. The optimum pH and thermal inactivation point were found to be 9, 6 and 75, 65°C for A and B isolates, respectively. Biologically characterization and morphology of phage particles confirmed two phage types, that can designated as phage MRSA-A and phage MRSA-B.
MRSA- phage, The spot test, Single plaque technique, Phage titer, Particle morphology
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