Twenty-four moderate halophilic isolates were obtained from excreta of wild Ass after enrichment in complete media broth and halophilic broth. All the isolates were Gram’s positive and non-capsulated. Further, isolates were screened for extracellular lipase secretion on solid media containing tributyrin as sole source of carbon and Mk-23 was potential lipase producer selected on the basis of zone index. Organism was identified as Halomonas salina on the basis of 16s r-RNA sequencing. Maximum lipase secretion was after 240 hours of incubation (During stationary phase). Organism was found to secrete maximum lipase at pH 6, 10% NaCl, 30°C temperature and 6% substrate concentration in liquid media.