Fluorescence helps in supervising events happening in epidermal. To detect the glucose level in the human body, a non-invasive method is proposed by exciting a target in its excited state. Preferred targets are Tryptophan of the human skin which is excited by fluorescence rays of a particular wavelength, and act as bio amplifiers or bio-reporters. The fluorescence  radiation  that  is  directed  to  the skin,  excites  the  glucose  containing components of Tryptophan. In turn, this helps to develop a clinically effective noninvasive technology that could help in accurate measurement over the blood glucose level.