ISSN: 0973-7510

E-ISSN: 2581-690X

Gazala H. Khan1, Arpit Gaur1, Asif B. Shikari1 , S. Najeeb2, G.A. Parray2, M.A. Ganai2, Ashaq Hussain2, Asif Iqbal2, Shafiq A. Wani3 and L.K. Sharma4
1Division of Genetics and Plant Breeding, India.
2Mountain Research Centre for Field Crops, Khudwani, India.
3Directorate of Research, 4Department of Genetics and Plant Breeding, Sher-e-Kashmir University of Agricultural Sciences and Technology of Kashmir Jammu and Kashmir, 191 121, India.
4Junagadh Agricultural University, Junagadh, – 362 001, India.
J. Pure Appl. Microbiol., 2016, 10 (1): 73-80
© The Author(s). 2016
Received: 14/11/2015 | Accepted: 02/01/2016 | Published: 31/03/2016
Abstract

Identification of restorers and maintainers from genetically diverse materials is a prerequisite for hybrid rice development. Generally restorers and maintainers are identified by evaluation of test cross hybrids for pollen and spikelet fertility. Phenotypic evaluation is a cumbersome, labour intensive and time consuming method. Also, in our previous studies the number of germplasm lines carrying traits for restoration seemed to be limited. Therefore, present study aimed to transfer Rf-4 gene from Basmati to one of our promising temperate background by employing Marker assisted backcross breeding. A genotype G-8 was crossed to elite WA-restorer line PRR-78 and were derived at least four lines carrying gene Rf-4 in BC1F2 with the help of gene linked marker RM6100. These lines were phenotyped for pollen and spikelet fertility restoration after crossing these in Line x tester fashion along with nine other lines with CMS lines SKAU-7A, SKAU-11A and IR68888A. Out of 13 lines two of the lines viz., PRR-78, SKAU-K-R-3-6-11 and SKAU-K-R-3-6-16 showed complete restoration coupled with high degree of heterosis for grain yield. The lines could be useful in development of three-line hybrids suitable for temperate high altitude areas beyond 1600 to 2000 m amsl.

Keywords

Rice, Hybrids, CMS, Restorer, Marker.

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© The Author(s) 2016. Open Access. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License which permits unrestricted use, sharing, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.