ISSN: 0973-7510

E-ISSN: 2581-690X

H.N. Shelat , P.K. Acharya, Y.K. Jhala and R.V. Vyas

1Department of Microbiology & Biofertilizer Project, B.A.College of Agriculture, Anand Agricultural University, Anand, Gujarat – 388110, India.
J. Pure Appl. Microbiol. 2014, 8(6):4809-4812
© The Author(s). 2014
Received: 20/08/2014 | Accepted: 30/10/2014 | Published: 31/12/2014

Liquid, Carrier and Granular formulations of Azotobacter chroococcum (ABA-1) were prepared from Jensen’s broth having 9.5×1011 cfu/ml . Bacterial survival in   formulations as well as on inoculated Pearl millet seeds was studied using Jensen’s agar  and there in vivo efficacy testing on growth parameters of P.millet cv GHB-558 in pots.  In formulations, Azotobacter count was  decreased 10 and 100 folds after 3 and 5 months, respectively. Survival of bacteria on seed was reduced from 1010 to 105 after 48 hours. Seed germination effect of all formulations on seed agar showed increased root and shoot length with secondary root formation as compared to uninoculated control. In a pot trial, Azotobacter formulation containing 2% glycerol + 2% PVP registered highest  plant height, shoot and root weight at 60 DAS by seed application in Pearl millet , which was significantly superior over other formulations, recommended dose (80 kg ha-1) of nitrogen and absolute control.


Azotobacter, Liquid formulation, Seed application, Pearl millet

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© The Author(s) 2014. Open Access. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License which permits unrestricted use, sharing, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.