ISSN: 0973-7510

E-ISSN: 2581-690X

Bin Rui1#, Han Wen1# , Yongkang Wang1, Wenfeng Wang2, Chen Zhang1, Kuanchao Zhang1, Yadong Fan1, Yifan Xie1 and Hongxian Zhang1
1School of Life Science, Anhui Agricultural University, 230026, Hefei, China.
2Plant protection institute, Anhui Agricultural University, 230026, Hefei, China.
J Pure Appl Microbiol. 2015;9(Spl. Edn. 1):115-122
© The Author(s). 2015
Received: 28/02/2015 | Accepted: 15/04/2015 | Published: 31/05/2015
Abstract

Along with the development of biotechnology, the effects of metabolites on the metabolic cycle and quantitative and qualitative analysis of biological metabolites is attracting more and more attention. We present a set of experiments that detect and quantitate the collision fragments of central carbon metabolism using liquid chromatography mass spectrometry to understand biological metabolites. In order to obtain a suitable method to use liquid chromatography mass spectrometry to detect the collision fragments of the central carbon metabolites, we first set the gradient elution parameters, and then determined whether to join the tributylamine in the sample solution. A good ion peak and a considerable response value were obtained using the gradient elution parameters in Table 2 and adding tributylamine in the sample solution. This was a suitable method for liquid chromatography mass spectrometry detection of the collision fragments of the central carbon metabolism. However, due to limited experimental conditions and time, exploration of other experimental parameters was not discussed in this study. Thus, the results of this experiment were obtained based on these limited experimental conditions.

Keywords

Liquid chromatography mass spectrometry; Metabolite; gradient elution; tributylamine; collision fragments

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