The aims of this study were to assess factors associated with KAPs, to evaluate grade shepherds and herdsmen’s knowledge about tick and microbiological agents transmitted by ticks as well as their prevention measures, to evaluate personal hygiene practices and to provide useful information for designing effective tick-borne microbiological diseases control programs. The descriptive study was carried out and interviewed in 66 herd’s including a total of 72 shepherds and herdsmen in 35 villages distributed all over the Qazvin province. Knowledge about tick-borne microbiological diseases, routes of their transmission, prevalence rate was unsatisfactory and this was significantly associated with the educational level (r£ 0.001). Most of them weren’t aware about such diseases. Nonparametric correlations test showed that knowledge of tick-borne microbiological diseases was significantly higher among young shepherds compared with olds (r£ 0.001). Health center and veterinary organizations staffs should disseminate relevant information about tick-borne microbiological diseases to help community members to be involved more in tick and tick-borne microbiological disease control.