ISSN: 0973-7510

E-ISSN: 2581-690X

Li Dai-Huan, Wei Dong-Zhi and Lin Jin-Ping
1State Key Laboratory of Bioreactor Engineering, NewWorld Institute of Biotechnology,
East China University of Science and Technology, Meilong Road 130, Shanghai 200237, China.
J Pure Appl Microbiol. 2014;8(2):1183-1189
© The Author(s). 2014
Received: 20/10/2013 | Accepted: 02/01/2014 | Published: 31/04/2014
Abstract

Optically pure (S)-1-phenyl-1,2-ethanodiol(PED) is a versatile chiral building block for the synthesis of pharmaceuticals, pheromones and liquid crystals. An alternative and convenient biocatalytic process was developed for the preparation of (S)-PED by Gluconobacter oxydans-catalyzed optical resolution of the corresponding racemate. Whole cells of G. oxydans DSM2003 were found to catalyze the regio- and stereo-selective concurrent oxidation of racemic PED to give (S)-PED with the optical purity of 99% and yield of 38% in 10h, meanwhile (R)-mandelic acid with moderate ee value produced. This oxidation is carried out by two sets of enzymes, the one made of membrane-bound alcohol dehydrogenase is responsible for the PED oxidation to hydroxyl aldehyde intermediate and the other composed of membrane-bound aldehyde dehydrogenase and other enzymes is responsible for the mandelic acid production.

Keywords

Gluconobacter oxydans, 1-phenyl-1,2-ethanodiol, Mandelic acid, Oxidative Resolution, Biocatalysis

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