ISSN: 0973-7510

E-ISSN: 2581-690X

Ramasamy Madhumathi and Manickam Velan
Department of Chemical Engineering, A.C. College of Technology, Anna University, Chennai – 600 025, India.
J. Pure Appl. Microbiol., 2016, 10 (1): 677-683
© The Author(s). 2016
Received: 14/11/2015 | Accepted: 12/01/2016 | Published: 31/03/2016
Abstract

Polyhydroxybutyrate (PHB) are polyesters of hydroxyalkanoates synthesized by various bacteria as intracellular carbon and energy storage compounds and accumulated as granules in the cytoplasm of cells. This biological macromolecule with wide range of applications in pharmaceutical, packaging, and different personal hygiene products. The present study deals with the enhanced production of PHB producing bacteria from soil were isolated, characterized and screened by Sudan black and Nile red staining methods. Screened organisms were subjected to the basis of morphological, biochemical and 16S rRNA analysis, the isolates were provisionally identified as Bacillus safensis EBT1 (JX679410), Thermus thermophilus EBT2 (KC684277), Bacillus halodurans EBT3 (KC008602), Rhodocyclus purpureus EBT5 (KC768770) and Bacillus megaterium EBT7 (JX870045). The strain EBT1 showed higher amount of PHB accumulation when compared to the other strains and was comparable with that of the other isolated strains. The PHB inclusions in cells were identified and affirmed using Fluorescent microscopy and FTIR analysis respectively.

Keywords

Polyhydroxybutyrate, Isolates, Sudan black, Nile red, Fluorescent microscopy.

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© The Author(s) 2016. Open Access. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License which permits unrestricted use, sharing, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.